Supplementary information to “ Dynamics of initiation , termination and reinitiation of DNA translocation by the motor protein EcoR 124 I ”

Determination of the reinitiation time We define a single translocation event as the time from when the DNA end-to-end distance starts to decrease due to translocation activity until the point where the end-toend distance returns to its resting length due to termination of translocation. Considering only one of the translocation directions of a bidirectionally translocating enzyme, the reinitiation time Treini,1 = Tbind,HsdR + Tini is simply the time between the end of one translocation event and the start of new translocation event in that direction. The average measurement time to observe N1 translocation events 〈Tmeas〉 is then ( ) event,1 reini,1 N1